原創(chuàng) 吳昕揚 植物脅迫與抗逆研究

近日，沈陽農(nóng)大齊紅巖團隊在PCE發(fā)表了題為《Red Light Induces Powdery Mildew Resistance by Activating the Expression of CmLOX10 in Oriental Melon》的研究論文，揭示了紅光通過激活甜瓜中CmLOX10的表達誘導(dǎo)白粉病抗性。

白粉?。≒M）是植物栽培中一種嚴重的真菌病害，對甜瓜的產(chǎn)量和品質(zhì)有重要的危害。

脂氧合酶（LOXs）廣泛參與病原體應(yīng)激反應(yīng)。作者之前的研究表明，紅光（RL）可以誘導(dǎo)甜瓜幼苗對PM的防御。然而，LOXs是否參與這一過程尚不清楚。

在本研究中，我們發(fā)現(xiàn)LOX活性對于RL誘導(dǎo)的PM抗性至關(guān)重要，CmLOX10被確認為關(guān)鍵成員。作者對甜瓜cDNA庫進行了酵母單雜交（Y1H）篩選，使用CmLOX10啟動子，鑒定出了CmWRKY41和CmABL5。這兩種蛋白都能調(diào)節(jié)CmLOX10的表達，從而參與RL誘導(dǎo)的PM抗性過程。

此外，光敏色素B（phyB）在蛋白質(zhì)水平上與CmABL5相互作用，增強了其對CmLOX10的轉(zhuǎn)錄激活。而沉默CmphyB削弱了RL對PM抗性的誘導(dǎo)。

綜上，作者的研究闡明了RL誘導(dǎo)后CmLOX10參與PM抗性的激活機制，從而豐富了光與病原真菌之間相互作用網(wǎng)絡(luò)。

Powdery mildew (PM) is a serious fungal disease in plant cultivation, which is detrimental to the yield and quality of oriental melons. Lipoxygenases (LOXs) are widely involved in pathogen stress response. Our previous studies have indicated that red light (RL) can induce defense to PM in oriental melon seedlings. However, it remains unclear whether LOXs are involved in this process. In this study, we found that LOX activity is essential for RL-induced PM resistance, with CmLOX10 is identified as a key member. We conducted a yeast one-hybrid (Y1H) screening of oriental melon cDNA libraries using the CmLOX10 promoter, which led to the identification of CmWRKY41 and CmABL5. Both of these proteins can regulate the expression of CmLOX10, thereby participating in the RL-induced PM resistance process. Furthermore, phytochrome B (phyB) interacts with CmABL5 at the protein level, enhancing its transcriptional activation of CmLOX10. Silencing CmphyB undermines the induction of RL resistance to PM. Our study elucidates the activation mechanism of CmLOX10 involved in PM resistance following RL induction, thereby enriching the network of interaction between light and pathogenic fungi.